Dot and slot blotting of DNA - ResearchGate Dot and slot blotting are simple techniques for immobilizing bulk unfractionated DNA on a nitrocellulose or nylon membrane. Hybridization analysis can then be carried out to determine the relative ... Mini-PROTEAN II Electrophoresis Cell Instruction Manual Bio-Rad also recommends that all Mini-PROTEAN II cell components and accessories be cleaned with a suitable laboratory cleaner (such as Bio-Rad Cleaning Concentrate, catalog number 161-0722) and rinsed thoroughly with distilled water before use. WARNING: Do not use alcohol to clean the plastic parts of the Mini-PROTEAN II cell. Stain-free detection as loading control alternative to ... Female Wistar rats and Zucker obese and lean control rats were used in this study, in addition to Caco-2 cells cultured in a standard fashion. Liver samples and Caco-2 cells were homogenized in RIPA buffer and processed for Western blot . We ran a series of protein blots using TGX Stain-Free gradient precast gels (Bio-Rad). Western Blot Protocols - MilliporeSigma | Life Science
Bio Rad Dot Blot Manual - WordPress.com
Here we describe DNA slot blot repair assay for quantitative detection of NER activity using DNA damage specific antibodies such as anti-CPD and anti-6-4PP. Briefly, genomic DNA irradiated with UV was isolated from cells, and the genomic DNA was vacuum-transferred to a nitrocellulose membrane using a Bio-Dot SF microfiltration apparatus (Bio-Rad). and Bio-Dot SF Microfiltration Apparatus - Bio-Rad Bio-Dot ® and Bio-Dot SF Microfiltration Apparatus. ... modular unit converts Bio-Dot apparatus to Bio-Dot SF apparatus; for slot blot transfer ... Bio-Rad Releases ... Western Blot Protocol | Bio-Rad
Slot Blot Hybridization Protocol - Tramvia Napoli
Instruction Manual - University of Sydney photocopy, recording, or any information storage or retrieval system, without permission in writing from Bio-Rad Laboratories. Bio-Rad reserves the right to modify its products and services at any time. This user guide is subject to change without notice. Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors, or for any ... Western Blotting Protocol - Research | MCDB Western Blotting Protocol Protein Isolation: *Always make fresh working solutions before isolation of proteins 1.) Dilute 50X PI solution to 1X PI in TM buffer keeping the solution on ice 2.) Weigh certain amount of tissues and chop them into small pieces 3.) Keep the tissue on dry ice 4.) Immuno Slot Blot Assay - raffaeleruberto.com
Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell From ...
Introduction to Western Blotting | Immunoblotting | Bio-Rad Western blotting, also known as immunoblotting is a core technique in cell and molecular biology. Our guide provides an overview for performing successful Western blots Direct vs Indirect Detection - Immunodetection | Bio-Rad Western Blotting chapter covering antibody detection of the target protein using the one step protocol (direct detection) or two-step protocol (indirect detection).
INTRODUCTION Dot blotting is an important technique that is routinely used in research and diagnostic laboratories. Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.
Protein Blotting Guide - Wolfson Centre Home Page Protein Blotting Guide Theory and Products Transfer The first phase of protein blotting is the transfer step, which involves moving the proteins from a solution or gel and immobilizing them on a synthetic membrane support (blot). Proteins can be transferred to membranes using a number of methods but the most Bio-Dot SF Microfiltration Apparatus Instruction Manual The Bio-Dot SF blotting apparatus has an evenly spaced, slot shaped sample template for easy slot blot sample comparisons. Because the Bio-Dot SF apparatus focuses the applied samples in a thin line instead of a circle, this slot format makes it easy to use a densitometer to quantitate results. DNA Slot Blot Repair Assay —BIO-PROTOCOL Here we describe DNA slot blot repair assay for quantitative detection of NER activity using DNA damage specific antibodies such as anti-CPD and anti-6-4PP. Briefly, genomic DNA irradiated with UV was isolated from cells, and the genomic DNA was vacuum-transferred to a nitrocellulose membrane using a Bio-Dot SF microfiltration apparatus (Bio-Rad). Western Blot Protocol | Bio-Rad
Troubleshooting Quantitative Western Blots Hints and Tips